This thesis developed multifunctional 3D-printed scaffolds for repairing critical-size mandibular bone defects. Using bioactive ceramics, surface coatings, and prevascularization strategies, it promoted both osteogenesis and angiogenesis. Results show that combining geometry, materials, and biological signals enables synergistic tissue regeneration, offering less-invasive alternatives to autologous bone grafts.

Myelin enables efficient communication between nerve cells and is essential for cognition, movement, and sensation. In neurodegenerative diseases, myelin is lost, impairing daily life. This research uses stem cells, gene profiling, and gene editing to uncover why myelin fails—and how regenerating it could transform treatment.

Craniosynostosis occurs when skull sutures fuse too early, requiring risky surgeries. The researcher identified microRNA-200A as a key regulator of suture development. In mice lacking miR-200A, sutures fused prematurely, but adding extra miR-200A via gene therapy prevented fusion entirely. This breakthrough suggests a non-surgical future treatment for craniosynostosis.

SVAS (Supravalvular Aortic Stenosis) is a rare condition where the aorta loses elasticity, causing dangerous thickening and narrowing. Using stem-cell technology, the researcher converts skin cells into aortic smooth muscle cells to study the disease and test treatments. A promising compound restores elasticity-related structures, offering hope for future therapies and broader disease modelling.